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CALL FOR SAMPLES FROM HEALTHY GERIATRIC SHAR-PEI

Drs. Francesca Puppo, Elaine Remmers and Dan Kastner at the National Institutes of Health (NIH) have asked me to put out another request for samples. We need the owners of dogs that are 12 years of age or older and that have NO history of (or even suspicion of) Familial Shar-Pei Fever (FSF) events or Amyloidosis (i.e. no signs of kidney or liver problems) AND preferably dogs with no known immediate relatives with signs of either FSF or Amyloidosis (parents, siblings or offspring) to send 2 ml of EDTA whole blood to the NIH. As you all well know, the biggest problem is identifying "normal" so if you have such a Shar-Pei or know of one, PLEASE HELP! This could be crucial research in identifying the underlying cause of this autoinflammatory disorder. For statistical reasons, it would be best to have at least 20 dogs in this part of the study so - if you share your life with a such a wonderfully fortunate dog - please consider helping Shar-Pei that are suffering and the future of the whole breed.

For those of you with an understanding of molecular genetics: In order to identify a novel genetic basis for FSF and clarify the molecular basis of similar autoinflammatory syndromes in humans, the scientists at the NIH are going to perform homozygosity mapping and association studies using high-density single nucleotide polymorphism (SNP) panels arrayed on Affymetrix chips.

This is huge project and your help is needed for this to happen. We also need more Shar-Pei mixed breed dog samples from those with a history consistent with FSF.

Shipping fees via FedEx will be paid by the NIH but there is no money available to reimburse you for the blood draw by your veterinarian. Your cooperation is greatly appreciated. I am very grateful to all of the people who have so enthusiastically helped by providing samples from their dogs - these samples are being actively used and analyzed in the on-going research at the NIH and at Dr. Anne Avery's laboratory at Colorado State University and progress IS being made.

Please email me at wvc@warwick.net for the medical questionnaire and consent form which you will send along with the blood sample to the NIH. I will also provide you with Dr. Kastner's FedEx shipping account number and his address to which you will ship the sample on ice (please try to avoid shipping samples over the weekend).

Again, thank you all so very much!

Linda

Dr. Linda Tintle
Wurtsboro Veterinary Clinic
163 Sullivan Street, PO Box 910
Wurtsboro, New York 12790
845-888-4884, Fax 845-888-5479
wvc@warwick.net
www.wvc.vetsuite.com

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